background [prelab assignment (20 points); due at the beginning of lab]
1. briefly, describe the following [6 points; 2 points / item]
Beer's law; include relationship between absorption and % transmission
standard or calibration curve, and its application / use
2. run Beer's law simulation; include screen shot of completed simulation (i.e. your screen shot shows the filled-in standard curve, intensity of the unknown sample, & your calculations to determine [unknown]) [5 points]
3. sketch a data table (i.e. not calculations) for this lab containing entries for all required experimental measurements (i.e. review purpose & content of lab report) [4 points]
4. MSDS data on copper (II) nitrate [5 points]
5. review below "methods" section prior to lab; otherwise, you might be "lost" during the lab, since you won't know how to use the colorimeter; because, this issue won't be addressed during the prelab session (due to shorter block period)
generate a standard (or calibration) curve
compare the determination of the concentration of Cu2+ in an unknown sample (range: 25 - 250 mM) using an algebraic- versus graphical- method in conjunction with the preceding calibration curve.
aqueous solution of copper (II) nitrate
- 250 mM; dilute to generate known [Cu(NO3 )2 ] - to be used to generate the calibration curve
- unknown [Cu(NO3 )2 ]
colorimeter (a "cheap" spectrophotometer)
water; beakers; test tubes; micropipets; tissue paper
methods - use of probe device - record in your lab notebook, since you will use the colorimeter in a subsequent lab [see vodcast; may use your smart phone during lab to access the link]
content of the lab report [35 points]
data analysis [15 points]
figure 1: standard curves using Excel: include all wavelengths, i.e. colors, and equation & R2 value of the "best" color to use in the analysis
figure 2: based on preceding graph, using the "best" color (to analyze the data) and graph paper, do a "free-hand" drawing of the graph
determination of the [Cu2+ ] in your sample - show your work using both methods
- algebraic method: use results of Excel output in figure 1
- graphical method: use graph in figure 2 and draw imaginary lines to connect the value of the absorbance (on the vertical axis) to the graph then to the value of the concentration (on the horizontal axis).
statistical analysis (include interpretation of p-value) of class data
discussion [10 points]
is there a difference in the determination of [Cu2+ ] using both methods described in the purpose ? Support your conclusion based on statistics
what is the rationale of choosing a specific wavelength of light for your determination of [Cu2+ ] ? [hint: see prelab question 1 in the below cdf based simulation]
rationalize why blue light has the lowest absorbance by the Cu2+ solution
error analysis (in regards to a cuvette containing the sample) [10 points]
how could the presence of fingerprints on the cuvette affect your determination of [Cu(NO3)2] ? elaborate.
when would the volume of the sample in the cuvette affect your determination of [Cu(NO3)2] ? elaborate.
Beer's law: derivation / regression analysis [calculus based presentation]
for Excel 2007 tutorials to analyze the calibration curve, see vodcast (@ lab / stats tab)
simulations (requires cdf player plugin or mathematica (nb)): guided inquiry into exploring the considerations to select the wavelength to use in the Beer's law lab.